{"id":4488,"date":"2023-07-31T10:40:19","date_gmt":"2023-07-31T08:40:19","guid":{"rendered":"https:\/\/www.uranyless.com\/coloration-negative\/"},"modified":"2025-01-28T16:13:22","modified_gmt":"2025-01-28T15:13:22","slug":"coloration-negative","status":"publish","type":"page","link":"https:\/\/www.uranyless.com\/fr\/coloration-negative\/","title":{"rendered":"coloration n\u00e9gative"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"4488\" class=\"elementor elementor-4488 elementor-542\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b3887f5 elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b3887f5\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-635ee33\" data-id=\"635ee33\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-c84fb43 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c84fb43\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-extended\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-3903b32\" data-id=\"3903b32\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6bb0d73 elementor-widget elementor-widget-heading\" data-id=\"6bb0d73\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Coloration N\u00e9gative<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-503f5a0 elementor-widget elementor-widget-heading\" data-id=\"503f5a0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Coloration n\u00e9gative des particules de type viral (VLPs) du norovirus - Uranyless<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5ca3f6b elementor-widget elementor-widget-text-editor\" data-id=\"5ca3f6b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"text-decoration: underline;\"><strong>Nouveau focus pour l&rsquo;application de la coloration n\u00e9gative des virus.<\/strong><\/span><\/p>\n<p>Merci aux retours de nos utilisateurs d&rsquo;UranyLess \u00e0 travers le monde. Certains d&rsquo;entre eux, pour des applications sp\u00e9cifiques, ont indiqu\u00e9 que leurs r\u00e9sultats de coloration n\u00e9gative pour les virus \u00e9taient moyens \u00e0 m\u00e9diocres.<\/p>\n<p>Notre laboratoire de Recherche et D\u00e9veloppement vient de r\u00e9aliser des <strong>ajustements techniques<\/strong> sur l&rsquo;Uranyless, qui offrent d\u00e9sormais d&rsquo;excellents r\u00e9sultats pour toutes les applications en coloration positive ou n\u00e9gative. (Nous sommes toujours int\u00e9ress\u00e9s par vos retours, contactez-nous \u00e0 info@deltamicroscopies.com).<\/p>\n<p><em>Nous avons d\u00e9velopp\u00e9 cette application en collaboration avec un laboratoire allemand (avec des remerciements particuliers au Dr Andr\u00e9 DIESSNER)<\/em><br \/><em>Icon Genetics GmbH \u2013 D \u2013 06120 Halle \/ Saale.<\/em><\/p>\n<p><strong>Nous vous pr\u00e9sentons leurs r\u00e9sultats de coloration n\u00e9gative : <span style=\"text-decoration: underline;\">Norovirus.<\/span><\/strong><\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-a57771d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"a57771d\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-extended\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-686bb72\" data-id=\"686bb72\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2712ee2 elementor-widget elementor-widget-image\" data-id=\"2712ee2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/2024\/07\/icon-coloration-negative-high.jpg?strip=all&lossy=1&w=2560&ssl=1\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"icon-coloration-negative-high\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTcxOCwidXJsIjoiaHR0cHM6XC9cL3d3dy51cmFueWxlc3MuY29tXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wN1wvaWNvbi1jb2xvcmF0aW9uLW5lZ2F0aXZlLWhpZ2guanBnIn0%3D\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/icon-coloration-negative-high-qqxyagzu65ewiiqb0chk36azrsz3p7fjo7d8wigzsw.jpg?strip=all&lossy=1&w=1920&ssl=1\" title=\"icon-coloration-negative-high\" alt=\"negative staining of corona observation\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-86476f9\" data-id=\"86476f9\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6110578 elementor-widget elementor-widget-image\" data-id=\"6110578\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/2024\/07\/vlps-1.jpg?strip=all&lossy=1&w=2560&ssl=1\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"vlps-1\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTcxOSwidXJsIjoiaHR0cHM6XC9cL3d3dy51cmFueWxlc3MuY29tXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wN1wvdmxwcy0xLmpwZyJ9\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/vlps-1-qqxyakr6xhk1sykuee42d5cu5cgkjzuh0pz6tmbf40.jpg?strip=all&lossy=1&w=1920&ssl=1\" title=\"vlps-1\" alt=\"corona observation cells with negative staining 2\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-a0dee31 elementor-widget elementor-widget-text-editor\" data-id=\"a0dee31\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"MsoNormal\"><b style=\"\"><u style=\"\">Image description:<\/u><\/b>&nbsp;Electron micrographs of highly purified plant-produced&nbsp;<b style=\"\">norovirus virus-like particles (VLPs)&nbsp;&nbsp;<\/b>examined by transmission electron microscopy (Zeiss EM900, Carl Zeiss Microscopy, Jena, Germany).&nbsp; Samples were collected on formvar coated cooper grids and contrasted with UranyLess.&nbsp; Micrographs were taken with a SM-1k-120 Variospeed slow scan camera (TRS, Moorenweis, Germany) using the iTEM software from Olympus SIS (M\u00fcnster, Germany).<\/p>\n<p class=\"MsoNormal\"><b>Note technique importante<\/b> <i>:<\/i><i>le&nbsp;<b>pH<\/b>&nbsp;of the contrast reagent,&nbsp; plays an important role during your negative staining, always predict a more or less&nbsp;<b>neutral pH<\/b>,&nbsp; the acidity disrupts the spread of your particles (viruses, bacteria, liposomes, \u2026). Uranyless pH 6,8 , uranyle acetate pH 4 <br><b>During the preparation of your sample<\/b>&nbsp;(Production, purification, \u2026) there is sometimes sucrose, EDTA, buffers and others. the uranyless can react strongly with these additions and the contrast is too strong, in this case wash with distilled water just after spreading your sample drop on the film support of the grid and contrast afterwards. <\/i><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1df1469 elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1df1469\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f788ec4\" data-id=\"f788ec4\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c973e04 elementor-widget elementor-widget-heading\" data-id=\"c973e04\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">T6 Phage-Uranyless<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-0755e67 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0755e67\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-extended\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-981ddea\" data-id=\"981ddea\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2076117 elementor-invisible elementor-widget elementor-widget-text-editor\" data-id=\"2076117\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;_animation&quot;:&quot;fadeInDown&quot;}\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"MsoNormal\"><b><u>T6 Phage<\/u><\/b><\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-77c3739 elementor-widget elementor-widget-text-editor\" data-id=\"77c3739\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong><em><u>Pr\u00e9paration de l&rsquo;\u00e9chantillon selon le protocole suivant :<\/u><\/em><\/strong><\/p>\n<ul>\n<li>\u00c9talement du phage T6 sur une grille G300-Cu recouverte d\u2019un film formvar carbone. Ionisation pendant 1 minute <\/li>\n<\/ul>\n<p>Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&amp;D \u2013 Delta Microscopies \u2013 France).<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-1ba6e89\" data-id=\"1ba6e89\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c80088d elementor-widget elementor-widget-image\" data-id=\"c80088d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/phage-T6-1-qqxysn7iauagz4c0w79wal7t7zekijkq65f6x3izi8.png?strip=all&lossy=1&w=1920&ssl=1\" title=\"phage-T6-1\" alt=\"mouse kidnay negative staining\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Rein de souris<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-2ea3698\" data-id=\"2ea3698\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bfa52eb elementor-widget elementor-widget-image\" data-id=\"bfa52eb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/2024\/07\/phage-T6-4.png?strip=all&lossy=1&w=2560&ssl=1\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"phage-T6-4\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTczOCwidXJsIjoiaHR0cHM6XC9cL3d3dy51cmFueWxlc3MuY29tXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wN1wvcGhhZ2UtVDYtNC5wbmcifQ%3D%3D\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/phage-T6-4-qqxys2j24hi5vp628yc3rqfo5i8ht7amrb2id0dnb4.png?strip=all&lossy=1&w=1920&ssl=1\" title=\"phage-T6-4\" alt=\"phage T6 negative staining\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Invaginations basales \u2013 h\u00e9mi-desmosomes \u2013 lame basale : augmentation de la surface d'\u00e9change \u2013 Rein.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-17cf9d0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"17cf9d0\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-extended\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-485a99c\" data-id=\"485a99c\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8db351a elementor-widget elementor-widget-image\" data-id=\"8db351a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/2024\/07\/phage-T6-3.png?strip=all&lossy=1&w=2560&ssl=1\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"phage-T6-3\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTc0MiwidXJsIjoiaHR0cHM6XC9cL3d3dy51cmFueWxlc3MuY29tXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wN1wvcGhhZ2UtVDYtMy5wbmcifQ%3D%3D\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/phage-T6-3-qqxyt6y4ad1hqxjcoxt28y8hp2pa06r38v4dzwppvk.png?strip=all&lossy=1&w=1920&ssl=1\" title=\"phage-T6-3\" alt=\"negative staining phage t6\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Zone glom\u00e9rulaire \u2013 podocytes \u2013 p\u00e9dicelles \u2013 Rein.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-8bf6689\" data-id=\"8bf6689\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-debac1b elementor-widget elementor-widget-image\" data-id=\"debac1b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/2024\/07\/phage-T6-2.png?strip=all&lossy=1&w=2560&ssl=1\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"phage-T6-2\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTc0MCwidXJsIjoiaHR0cHM6XC9cL3d3dy51cmFueWxlc3MuY29tXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wN1wvcGhhZ2UtVDYtMi5wbmcifQ%3D%3D\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/phage-T6-2-qqxysfoss606e8my440vqn44gwfmsyqvh47b2vu4w0.png?strip=all&lossy=1&w=1920&ssl=1\" title=\"phage-T6-2\" alt=\"phage t6 picture 3 staining\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Podocytes \u2013 p\u00e9dicelles \u2013 Rein.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-c6844d2\" data-id=\"c6844d2\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-0ecdd22 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"0ecdd22\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3106677 elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3106677\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-246bbf2\" data-id=\"246bbf2\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6e1ff6f elementor-widget elementor-widget-heading\" data-id=\"6e1ff6f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">E.coli Bacteries - Uranyless<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-76d33a7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"76d33a7\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-extended\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-0678a83\" data-id=\"0678a83\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d462054 elementor-widget elementor-widget-text-editor\" data-id=\"d462054\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>UranyLess negative staining during<strong> 2 minutes <\/strong>of E. Colu bacteries type (adherent and invasive (AIEC) LF82) which produce pili and flagella, team M2iSH from Clermont-Ferrand, France.<\/p>\n<p>Observation au microscope \u00e9lectronique en transmission \u00e0 80 kV.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-f892105\" data-id=\"f892105\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-703e6cb elementor-widget elementor-widget-image\" data-id=\"703e6cb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/2024\/07\/CN-Bacterie-2Clermont.png?strip=all&lossy=1&w=2560&ssl=1\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"CN-Bacterie-2Clermont\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTc0NywidXJsIjoiaHR0cHM6XC9cL3d3dy51cmFueWxlc3MuY29tXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wN1wvQ04tQmFjdGVyaWUtMkNsZXJtb250LnBuZyJ9\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/CN-Bacterie-2Clermont-qqxz08qjjmowtnapiziby66y33ydqgqm7r9hkm9f7k.png?strip=all&lossy=1&w=1920&ssl=1\" title=\"CN-Bacterie-2Clermont\" alt=\"bacterie negative staining\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-3ef8a76\" data-id=\"3ef8a76\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4fc6660 elementor-widget elementor-widget-image\" data-id=\"4fc6660\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/2024\/07\/CN-Bacterie-Clermont.png?strip=all&lossy=1&w=2560&ssl=1\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-title=\"CN-Bacterie-Clermont\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MTc0OCwidXJsIjoiaHR0cHM6XC9cL3d3dy51cmFueWxlc3MuY29tXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wN1wvQ04tQmFjdGVyaWUtQ2xlcm1vbnQucG5nIn0%3D\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/evcchbemxkq.exactdn.com\/wp-content\/uploads\/elementor\/thumbs\/CN-Bacterie-Clermont-qqxz0g992az7eizsb2rci4amu6xbg1kgwshdety9ts.png?strip=all&lossy=1&w=1920&ssl=1\" title=\"CN-Bacterie-Clermont\" alt=\"bacterie negative staining\" class=\"elementor-animation-float\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-29fc2ae elementor-section-content-top elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"29fc2ae\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;mdp_selection_sticky_effect_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-25 elementor-top-column elementor-element elementor-element-c77092a\" data-id=\"c77092a\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-97f226f elementor-widget elementor-widget-image\" data-id=\"97f226f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" 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class=\"elementor-icon-list-text\">Uranyless<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.uranyless.com\/lead-citrate\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Citrate de plomb<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.uranyless.com\/stain-77-2\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Stain 77<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.uranyless.com\/application\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Applications<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.uranyless.com\/blog\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Blog<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-25 elementor-top-column elementor-element elementor-element-6682d86\" data-id=\"6682d86\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;mdp_selection_sticky_column_effect_enable&quot;:false}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b8bc306 elementor-widget elementor-widget-heading\" data-id=\"b8bc306\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 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Merci aux retours de nos utilisateurs d&rsquo;UranyLess \u00e0 travers le monde. 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